Table 2. Primers and probes employed in this study.
| Assay type | Primer name | Oligonucleotide (5’-3’) | Reference |
|---|---|---|---|
| TevRPA |
TevRPA-Fw TevRPA-Rv |
CACCGAAGCAAGCGCAGCAAGAGGGTTAGCA GTAGCTGTCTCCTGGGGCCGAGGTGTCATAG |
This study |
| TevRPA-LF |
TevRPA-Rv-biotin FAM-Probe 1 FAM-Probe 2 |
[Biotin]GTAGCTGTCTCCTGGGGCCGAGGTGTCATAG [6F]TCTGCCCGCAGTTGCCTATGGCGGCGAAGT[dS]GCAGGGGCGATTTCAT[C3] [6F]CTAAAATTTCTAAAGCACGCGGTTGGCAACA[dS]CAAGTTTGTGTGGGC[C3] |
This study |
| PCR | RoTat1.2 Fw RoTat1.2 Rv |
GCGGGGTGTTTAAAGCAATA ATTAGTGCTGCGTGTGTTCG |
[40] |
6F stands for 6FAM, dS for dSpacer, and C3 for C3-spacer.