Fig 7. AP2-associated clathrin is mislocalized in NEKL-depleted adults.
(A–F) Representative images of (A,D) GFP::CHC-1, (B,E) Phyp7mScarlett::DPY-23/μ2, and merged images (C,F) in auxin-treated (A–C) wild-type and (D–F) NEKL-3::AID adults. mScarlet is represented as magenta, overlap is white. (A’–F’) Magnified inserts of indicated regions in (A–F). Bar sizes in A and A’ = 5 μm (for A–F and A’–F’). (G) Pearson’s r coefficients are shown for the indicated strains; circles correspond to images from individual worms. (H,I) Mean intensities and the percentage of positive pixels above threshold were calculated for (H) GFP::CHC-1 and (I) mScarlet::DPY-23/μ2 for auxin-treated wild-type and NEKL-3::AID adults. p-Values were determined using two-tailed Mann-Whitney tests; ****p < 0.0001, *p < 0.05. Raw data are available in S1 File.