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. 2020 Feb 18;16(2):e1008633. doi: 10.1371/journal.pgen.1008633

Fig 11. NEKLs and NCAP-1 likely affect trafficking through distinct mechanisms.

Fig 11

(A) Representative confocal images of day-2 adults expressing GFP::CHC-1 within the apical region of the hyp7 epidermal syncytium. Background subtraction was performed using the same parameters for all images; minimum and maximum pixel values were kept consistent for all images. Inverted fluorescence was used to aid clarity. Bar size in A = 5 μm. (B,C) For individual adults, the mean GFP::CHC-1 intensity (B) and the percentage of GFP-positive pixels above threshold (C) were determined. The group mean and 95% confidence interval (error bars) are shown. p-Values were determined using two-tailed Mann-Whitney tests; *p < 0.05. (D,E) Fluorescence recovery curves of GFP::CHC-1 after photobleaching showing ncap-1(mew39) (D) and dpy-23(mew74) (E) day-2 adults. Normalized average mean intensities of photobleached regions were plotted as a function of time using -5 s intervals; error bars denote SEM. (F) Bar plot showing the percent mobile fraction from FRAP analyses; error bars indicate 95% confidence intervals. Raw data are available in S1 File.