FIG. 2.

Expression of chick Six3 protein and RNA in the eye anterior segment. Immunocytochemical staining of Six3 protein (B, D–G) and Pax6 protein (A, C) is shown. Arrowheads point to tissues expressing either both proteins (black) or Six3 protein only (green). The anti-Six3 antibodies used in (E) and (F) have been preincubated with the Six3 virus-infected and the RCAS virus-infected DF1 cells, respectively. (H) Results of RT-PCR amplification of Six3, Six6, Pax6, Pax2, and G3PDH (control) using E6 and E12 corneal cDNAs, E6 retina, and pigmented epithelium cDNAs (r/p), or corneal RNAs without reverse transcription. Red asterisks (*) indicate the expected positions of amplified products. Scale bars, 100 μm. Abbreviations: cor, cornea; le, lens; ir, iris; tbm, trabecular meshwork.