a, Quantification of SCFAs in SUP of
F. PB1 and L. lactis by UPLC-MS. Data
from 2 or 6 independent experiments (L. lactis SUP n = 2;
F. PB1 SUP n = 6 biologically independent experiments).
b,c, ApcMin/+ mice received vehicle (Veh, n = 7
mice/group), F. PB1 or L. lactis (n = 8
mice/group) from week 8 to 10. b,Tumor multiplicity in the
small intestine normalized to vehicle treated ApcMin/+ mice.
c, Area and maximum diameter (axis length) of ileal
dysplastic lesions normalized to the total number of lesions per mouse. Box
plots show the interquartile range, median value and whiskers min to max.
d, qPCR of L. lactis abundance normalized
to panbacterial primers targeting the 16S rRNA gene (UNI 16S) in bacterial
DNA extracted from feces (both at time 0 and 48h after last gavage) and
mucus from the ileum and colon of WT and ApcMin/+ mice pretreated
or not with antibiotics (ABX) and then monocolonized with L.
lactis (n = 4 mice/group). e,f, 11 weeks old
ApcMin/+ mice treated with broths not fermented (Veh) or
fermented by L. lactis (SUP) in the presence of ABX (n = 6
mice/group). e, Tumor multiplicity in the small intestine
normalized to vehicle treated ApcMin/+ mice. f, Area
and maximum diameter of ileal dysplastic lesions normalized to the total
number lesions per mouse. Box plots show the interquartile range, median
value and whiskers min to max. g, Cell proliferation assay on
mouse CRC cell lines treated or not with sodium lactate at different
concentrations for 48h. t0 is the signal from cells at the time of
stimulation. Two independent experiments were performed with consistent
results. Data presented as means ± s.d. of a representative
experiment (n = 6 biologically independent samples). P
values were determined by two-tailed unpaired Mann-Whitney test
(e,f), one-way ANOVA with Bonferroni post-test
(b,g) or Kruskal-Wallis test with Dunn post-test
(c,d). Data are presented as means ± s.e.m. in
a-f.