a, qPCR of mucin expression in the ileal tissue of WT mice and
healthy (H) and tumor (T) tissue of ApcMin/+ mice at 8 and 16 weeks
of age. Expression levels normalized to the reference gene Rpl32. Data from two
independent experiments (8wks: WT n = 10, ApcMin/+ H n = 8,
ApcMin/+ T n = 7; 16 wks: WT n = 8, ApcMin/+ H n = 9,
ApcMin/+ T n = 9 mice/group). P values were
determined by one-way ANOVA with Bonferroni post-test to compare expression
levels within the same time point. b,c, F. PB1
administration experiments in WT and ApcMin/+ mice pre-treated with
antibiotic cocktail. b, qPCR of F. PB1 abundance
normalized to panbacterial primers targeting the 16S rRNA gene (UNI 16S) in
bacterial DNA extracted from ileal mucus. Data from two independent experiments
(WT Veh n = 7; WT F. PB1 n = 10; ApcMin/+ Veh n = 6;
ApcMin/+
F. PB1 n = 11 mice/group). P values were
determined by two-tailed unpaired Mann-Whitney test. c,
Representative FISH images of F. PB1 (green) on the mucosal
surface of ApcMin/+ ileum polyp and WT normal ileum. DAPI nuclear
stain in blue. Images obtained at 40X magnification, scale bars 50 μm; n
= 3 mice/group. d, Tumor multiplicity in the small intestine of
ApcMin/+ mice treated with vehicle (Veh) or F.
PB1 from week 4 to 8 (n = 5 mice/group). Significance determined by multiple
t-tests corrected for multiple comparisons using the
Holm-Sidak method to compare tumor multiplicity between groups at each time
point. e,f, ApcMin/+ mice received vehicle (Veh) or
F. PB1 from week 8 to 12. Two independent experiments were
performed with consistent results. e, Tumor multiplicity in the
small intestine normalized to vehicle treated ApcMin/+ mice at 12
weeks of age. Data from two independent experiments (n = 14 mice/group).
f, Area and maximum diameter (axis length) of ileal dysplastic
lesions normalized to the total number of lesions per mouse. Data from one
representative experiment (n = 7 mice/group). Box plots show the interquartile
range, median value and whiskers min to max. P values were
determined by two-tailed unpaired t-test (e) and
two-tailed unpaired Mann-Whitney test (f). a,b,d-f,
Data are represented as means ± s.e.m.