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. Author manuscript; available in PMC: 2020 Jul 27.
Published in final edited form as: Nat Microbiol. 2020 Jan 27;5(3):511–524. doi: 10.1038/s41564-019-0649-5

Fig. 5. F. PB1 metabolic products, in particular butyrate, have anti-proliferative activity in vivo and this is independent on the microbiota.

Fig. 5

a,b, ApcMin/+ mice received broths not fermented (Veh) or fermented by F. PB1 (SUP) from week 8 to 10. Data from three independent experiments (Veh n = 13; SUP n = 11 mice/group). c-e, 11 weeks old ApcMin/+ mice treated with Veh or F. PB1 SUP in the presence of an antibiotic cocktail (ABX). Data from two independent experiments (Veh n = 6; SUP n = 7 mice/group). f,g, 11 weeks old ApcMin/+ mice treated with Veh, F. PB1 SUP or butyrate 1 mM in the presence of ABX. Data from two independent experiments (Veh, n = 7; SUP and Butyrate n = 8 mice/group). a,c,f, Tumor multiplicity in the small intestine normalized to vehicle treated ApcMin/+ mice. b,d,g, Area and maximum diameter of ileal dysplastic lesions normalized to the total number lesions per mouse. e, Representative images of ileal dysplastic lesions stained with anti-NFATc3 antibody (200X magnification, scale bars 100 μm) or with anti-Histone H3 acetyl K27 antibody (100X magnification, scale bars 200 μm). Right panel: Quantitative color deconvolution analysis of Histone H3 acetylation in dysplastic lesions of ApcMin/+ mice treated or not with F. PB1 SUP (Veh, n = 6; SUP, n = 4 biologically independent samples). h,i, AOM/DSS treated C57BL/6 WT mice received Veh (n = 5 mice/group) or F. PB1 SUP (n = 6 mice/group) Data from one representative experiment depicted. h, Tumor multiplicity in the colon normalized to vehicle treated ApcMin/+ mice. i, Area and maximum diameter (axis length) of colon adenomas normalized to the total number of lesions per mouse. Two (c-i) or three (a,b) independent experiments were performed with consistent results. a-i, Data are represented as means ± s.e.m. and box plots show the interquartile range, median value and whiskers min to max in b,d,g,i. P values were evaluated using two-tailed unpaired Mann Whitney test (a-e,h), Kruskal-Wallis test with Dunn post-test (f,g) or two-tailed unpaired t-test (i).