a,b, ApcMin/+ mice received broths not fermented (Veh) or
fermented by F. PB1 (SUP) from week 8 to 10. Data from three
independent experiments (Veh n = 13; SUP n = 11 mice/group). c-e,
11 weeks old ApcMin/+ mice treated with Veh or F.
PB1 SUP in the presence of an antibiotic cocktail (ABX). Data from two
independent experiments (Veh n = 6; SUP n = 7 mice/group). f,g, 11
weeks old ApcMin/+ mice treated with Veh, F. PB1 SUP
or butyrate 1 mM in the presence of ABX. Data from two independent experiments
(Veh, n = 7; SUP and Butyrate n = 8 mice/group). a,c,f, Tumor
multiplicity in the small intestine normalized to vehicle treated
ApcMin/+ mice. b,d,g, Area and maximum diameter of
ileal dysplastic lesions normalized to the total number lesions per mouse.
e, Representative images of ileal dysplastic lesions stained
with anti-NFATc3 antibody (200X magnification, scale bars 100 μm) or with
anti-Histone H3 acetyl K27 antibody (100X magnification, scale bars 200
μm). Right panel: Quantitative color deconvolution analysis of Histone H3
acetylation in dysplastic lesions of ApcMin/+ mice treated or not
with F. PB1 SUP (Veh, n = 6; SUP, n = 4 biologically
independent samples). h,i, AOM/DSS treated C57BL/6 WT mice received
Veh (n = 5 mice/group) or F. PB1 SUP (n = 6 mice/group) Data
from one representative experiment depicted. h, Tumor multiplicity
in the colon normalized to vehicle treated ApcMin/+ mice.
i, Area and maximum diameter (axis length) of colon adenomas
normalized to the total number of lesions per mouse. Two (c-i) or
three (a,b) independent experiments were performed with consistent
results. a-i, Data are represented as means ± s.e.m. and box
plots show the interquartile range, median value and whiskers min to max in
b,d,g,i. P values were evaluated using
two-tailed unpaired Mann Whitney test (a-e,h), Kruskal-Wallis test
with Dunn post-test (f,g) or two-tailed unpaired
t-test (i).