a, Shannon diversity index in fecal DNA from healthy donors (n = 61)
and large adenoma (n = 15) patients and abundance of the family
Erysipelotrichaceae. Box plots show the interquartile
range, median value and whiskers min to max. b, Abundance of the
undefined genus Erysipelotrichaceae noname and of the species
Holdemanella biformis. At each taxonomic level, a
two-tailed Wilcoxon Rank-Sum test comparing relative abundances of large adenoma
(n = 15) and control samples (n = 61) was applied. P-values
obtained at family and genus taxonomic levels were corrected for multiple
hypothesis testing using the Benjamin-Hochberg procedure. c, A
high-quality phylogeny of the Erysipelotrichaceae family and
the F. PB1 isolate. d, Quantification of SCFAs in
the broth fermented by Holdemanella biformis (H.
biformis SUP) by UPLC-MS. Data from two independent experiments (n
= 2 biologically independent experiments). e, Representative WB
from two to three independent experiments performed with consistent results
showing the effect of SCFAs MIX, F. PB1 SUP, H.
biformis SUP on H3K27 acetylation and NFATc3 expression in human
CRC cell lines. Cells not treated (NT) or treated with non-fermented medium
(Veh) as a control. Vinculin used as loading control. Densitometric analysis is
reported in Extended Data Fig. 8b.
f,g, 11 weeks old ApcMin/+ mice treated with Veh or
H. biformis SUP in the presence of antibiotics (ABX) (n = 5
mice/group). f, Tumor multiplicity in the small intestine
normalized to vehicle treated ApcMin/+ mice. g, Area and
maximum diameter of ileal dysplastic lesions normalized to the total number
lesions per mouse. Box plots show the interquartile range, median value and
whiskers min to max. h, Representative WB from two (H.
biformis SUP) to three (F. PB1 SUP) independent
experiments performed with consistent results showing the effect of
F. PB1 SUP or H. biformis SUP on H3K27
acetylation and NFATc3 expression in ex-vivo treated human
colon tumor samples (hCRC). Bar plots show the densitometric quantification of
NFATc3 (normalized to vinculin) and H3K27 acetylation (normalized to total H3)
(H. biformis SUP, n = 2; F. PB1 SUP, n = 3
biologically independent experiments). Data are represented as means ±
s.e.m. in a,b,d,f-h. P values were evaluated using
two-tailed unpaired Mann Whitney test (a,f,g right panel) and
two-tailed unpaired t-test (g left panel,
h).