a, Representative Western blots of two independent
experiments with consistent results showing the abundance of Muc1, Muc13 and
Muc20 in ileal extracts from WT (healthy tissues, H) and healthy (H) and
tumor (T) tissues of ApcMin/+ mice analyzed at 11 weeks of age;
Right: densitometric quantification (WT n = 3; ApcMin/+ n = 4
biologically independent samples). b, Representative confocal
images of WT and ApcMin/+ mice ileum intestinal tissues stained
with Muc1 (red) and DAPI (blue); scale bars, 50 μm. Mean fluorescence
intensity of MUC1 was measured to quantify the expression of the protein in
the intestinal of ApcMin/+ and WT mice (WT n = 7,
ApcMin/+ n = 5 biologically independent samples).
c, ApcMin/+ mice were treated with vehicle (n =
10 mice/group) or F. PB1 (n = 11 mice/group) from week 8 to
10. Number of microscopic lesions, shown as % relative to vehicle, was
evaluated on H&E slides by an expert pathologist. a-c,
Data from two independent experiments and represented as means ±
s.e.m.. P values were determined by one-way ANOVA using
Tukey post-test (a), two tailed unpaired Mann Whitney test
(b), or two-tailed unpaired t-test
(c).