a-c, Flow cytometry analyses of the small intestinal
and colonic lamina propria of germ-free ICR mice monocolonized with
F. PB1 (GF + F. PB1 n = 10 mice/group)
for the presence of T reg (a), Th17 (b) and Th1
(c) cells. Germ-free (GF, n = 9 mice/group) and SPF (n = 7
mice/group) mice were used as controls. d-g WT and
ApcMin/+ mice treated with vehicle (Veh) or
F. PB1 from week 8 to 12. d,e, Flow
cytometric analysis of T reg, Th1 and Th17 cell populations in the small
intestinal lamina propria. FoxP3+CD25+ are gated on the live CD45+ CD3+ CD4+
cells; Helios+ is gated on the FoxP3+ CD25+ cells (WT Veh,
ApcMin/+
F. PB1 n = 12; WT F. PB1 n = 14;
ApcMin/+ Veh n = 11 mice/group). IL17+, IFNγ+ and
IL17+ IFNγ+ cells are gated on the live CD45+ CD3+ CD4+ cells (WT
Veh, ApcMin/+ Veh n = 9; WT F. PB1 n = 11;
ApcMin/+
F. PB1 n = 10 mice/group). Data shown as % of CD45+ cells
(d) or as absolute number / whole tissue (e).
f,g, Flow cytometric analysis of mononuclear phagocytes
(CD11b+F4/80+ macrophages, CD11c+CD11b+ dendritic cells and
Ly6ChiCD11b+ inflammatory monocytes) and neutrophils
(Ly6G+CD11b+) in the small intestinal lamina propria (WT Veh n = 5; WT
F. PB1, ApcMin/+
F. PB1 n = 6; ApcMin/+ Veh n = 4). Data shown as
percentages relative to the CD45+ CD3- population (f) or as
absolute number / whole tissue (g). h, Flow
cytometric analysis of peripheral blood cells. Data shown as absolute number
/ ml blood (WT Veh, ApcMin/+
F. PB1 n = 13; WT F. PB1 n = 15;
ApcMin/+ Veh n = 12 mice/group) P values
were determined by one-way ANOVA with Bonferroni post-test
(a-d), Kruskal-Wallis with Dunn post test (e),
two-tailed unpaired Mann-Whitney test (f,g,h Ly6G+CD11b+) or
two-tailed unpaired t-test (h,
Ly6ChiCD11b+). a-h, Data are represented as
means ± s.e.m..