Extended Data Fig. 3. F. PB1 does not have a major impact on immune cells.

a-c, Flow cytometry analyses of the small intestinal and colonic lamina propria of germ-free ICR mice monocolonized with F. PB1 (GF + F. PB1 n = 10 mice/group) for the presence of T reg (a), Th17 (b) and Th1 (c) cells. Germ-free (GF, n = 9 mice/group) and SPF (n = 7 mice/group) mice were used as controls. d-g WT and Apc^Min/+ mice treated with vehicle (Veh) or F. PB1 from week 8 to 12. d,e, Flow cytometric analysis of T reg, Th1 and Th17 cell populations in the small intestinal lamina propria. FoxP3+CD25+ are gated on the live CD45+ CD3+ CD4+ cells; Helios+ is gated on the FoxP3+ CD25+ cells (WT Veh, Apc^Min/+ F. PB1 n = 12; WT F. PB1 n = 14; Apc^Min/+ Veh n = 11 mice/group). IL17+, IFNγ+ and IL17+ IFNγ+ cells are gated on the live CD45+ CD3+ CD4+ cells (WT Veh, Apc^Min/+ Veh n = 9; WT F. PB1 n = 11; Apc^Min/+ F. PB1 n = 10 mice/group). Data shown as % of CD45+ cells (d) or as absolute number / whole tissue (e). f,g, Flow cytometric analysis of mononuclear phagocytes (CD11b+F4/80+ macrophages, CD11c+CD11b+ dendritic cells and Ly6C^hiCD11b+ inflammatory monocytes) and neutrophils (Ly6G+CD11b+) in the small intestinal lamina propria (WT Veh n = 5; WT F. PB1, Apc^Min/+ F. PB1 n = 6; Apc^Min/+ Veh n = 4). Data shown as percentages relative to the CD45+ CD3- population (f) or as absolute number / whole tissue (g). h, Flow cytometric analysis of peripheral blood cells. Data shown as absolute number / ml blood (WT Veh, Apc^Min/+ F. PB1 n = 13; WT F. PB1 n = 15; Apc^Min/+ Veh n = 12 mice/group) P values were determined by one-way ANOVA with Bonferroni post-test (a-d), Kruskal-Wallis with Dunn post test (e), two-tailed unpaired Mann-Whitney test (f,g,h Ly6G+CD11b+) or two-tailed unpaired t-test (h, Ly6C^hiCD11b+). a-h, Data are represented as means ± s.e.m..