Figure 4.

A single restraint stress reduces PACAP/PAC1 regulation of the CeM GABA network. A. 5 nM PACAP-38-induced facilitation of the sIPSC frequency was lost after a single restraint stress, but recovered toward naïve levels with repeated restraint stress sessions (6–9 cells from 3–5 rats per group). The data from naïve rats shown here are the same as in Fig. 2C. B. 250 nM PACAP(6–38) reduced the sIPSC frequency in naïve rats and repeated restraint stress rats, but not in animals subjected to a single restraint stress (5–8 cells from 3–4 rats per group). The data from naïve rats shown here are the same as in Fig. 2E. C. The loss of PACAP-38’s ability to facilitate GABA release after a single restraint stress persisted with higher agonist concentrations (25 nM or 50 nM; 4–9 cells from 3–5 rats per group). The 5 nM PACAP-38 data are the same as in Fig. 2C. All data are presented as mean±SEM. *p<0.05 and **p<0.01 by one sample t-test; ^$p<0.05 by one-way ANOVA with post hoc Tukey’s multiple comparisons test; ^&&&p<0.001 by two-way ANOVA with post hoc Tukey’s multiple comparisons test indicating a significant difference between the single restraint stress group as compared with the naive and repeated restraint stress groups.