Figure 3.
Electrochromic voltage-sensitive dye Di-4-ANEPPS applied to hiPSC-CMs from 2 commercial sources exposed to increasing concentrations of an inhibitor of the repolarizing hERG potassium channel. Clear measurement of increases in AP duration and early after depolarizations (EADs) is possible with the voltage-sensitive dye. “Ratiometric” optical recordings show ratio of two emission wavelength bands from the dye. A. Cor4U hiPSC-CMs. B. iCell hiPSC-CMs. Reprinted with modification from original (Hortigon-Vinagre et al., 2016, Fig. 7).