Primary neurons were treated with vehicle (DMSO) or GOT enzyme inhibitor, Aminooxyacetic acid (AOA), for 48 hours. OCR and ECAR were assessed using the Seahorse Biosciences Technology. Values were normalized to cell counts and represented as fold change of DMSO control. (a, b) Measurements and quantifications of OCR with (a) 20 µM and (b) 80 µM DMSO or AOA. (c, d) Measurements and quantifications of ECAR with (c) 20 µM, and (d) 80 µM DMSO or AOA (n=4–5, mean ± SEM, * P <0.05, ** P <0.01, Two-tailed paired Student’s t-test).