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. Author manuscript; available in PMC: 2021 Jun 1.
Published in final edited form as: Transl Stroke Res. 2019 Aug 31;11(3):418–432. doi: 10.1007/s12975-019-00729-4

Fig. 7. Schematic diagram of the experimental design.

Fig. 7

Briefly, (a, b) synaptosomes were extracted from adult rats. Phosphoproteomic analysis, immunoprecipitation and immunoblots were used to examine PKCε-activated phosphorylation. (c-e) Primary neurons were prepared from embryonic 18–20 day old pups. Oxygen consumption and glycolysis were evaluated by Seahorse Bioscience Technology. PKCε-mediated phosphorylation was determined by immunoprecipitation and western blots. A colorimetric assay was utilized to assess the GOT2 activity. We used oxygen-glucose deprivation as an in vitro model to evaluate the effects of ischemic injury on GOT2 activity. For pharmacological treatments and experimental details please refer to “Materials and Methods”.