Figure 5.

An intact glycocalyx protects cholangiocytes from bile acids. (A‐D) Staining using the lectins, SNA and SBA, in the bile duct‐on‐a‐chip (A,B) before and (C,D) after neuraminidase treatment. Scale bars, 100 μm. (E) Schematic showing that SNA recognizes sialyated carbohydrates, and that removal of sialic acid is required for recognition by SBA. (F) Diffusive permeability of devices treated with the bile acid, GCDC, with or without previous desialyation with neuraminidase, n = 4‐6 devices for each condition. All data are presented as mean ± SD; *P < 0.05.