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. 2019 Oct 28;71(4):1350–1363. doi: 10.1002/hep.30918

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© 2019 The Authors. Hepatology published by Wiley Periodicals, Inc., on behalf of American Association for the Study of Liver Diseases.

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes.

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Bile duct‐on‐a‐chip with primary murine extrahepatic cholangiocytes. Immunofluorescence images across the bottom (left panels) and middle (right panels) of channels stained with antibodies (shown in red or green) against (A) K19, (B) ASBT, (C) E‐cadherin, (D) ZO‐1, and (E,F) acetylated α‐tubulin, with DAPI nuclear staining (blue). Top (left panel, F) and cross‐sectional (right panel, F) views of cilia (white arrow) in the cholangiocyte channel. Scale bars, 50 μm (left panels, except F); 100 μm (right panels and F, left panel). Images are representative of three independent experiments.