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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Hepatology. 2020 Jan 24;71(4):1228–1246. doi: 10.1002/hep.30921

Figure 4. Cell-autonomous regulation of hepatocyte signaling and gene expression by hnRNPU.

Figure 4.

The following experiments were performed in primary hepatocytes prepared from Ctrl or LKO mice. Hepatocytes were cultured in serum-free media overnight before treatments. PA (200 μM) was added 2 hrs prior to the addition of TNFα (20 ng/mL).

(A) Immunoblots of total lysates from hepatocytes treated with vehicle (−) or PA+TNFα (+) for 15 min.

(B) Immunoblots of cytosolic or nuclear extracts from treated hepatocytes.

(C) Immunoblots of total lysates from hepatocytes treated with vehicle (−) or PA+TNFα (+) for 24 hrs.

(D) qPCR analysis of gene expression from hepatocytes treated with vehicle (Veh) or PA+TNFα for 6 hrs (n=3). Data represent mean ± SEM. **P<0.01, Ctrl vs. LKO in vehicle-treated cells. #P<0.05, ##P<0.01, Ctrl vs. LKO in hepatocytes treated with PA+TNFα. Two-way ANOVA with multiple comparisons.