Fig. 1.

P. aeruginosa’s LPS-induced EMT in BEAS-2B cells. BEAS-2B cells were incubated with 2 μg/mL P. aeruginosa’s LPS for 24, 48, or 72 h. Western blotting revealed that LPS decreased the expression of E-Cad and upregulated the expression of Vi and α-SMA in a time-dependent manner (a). Immunofluorescence showed similar results of the upregulated mesenchymal marker Vi and downregulation of the epithelial marker, E-Cad, in a time-dependent manner (b). Compared with the basal condition, a fibroblast-like, spindle-shaped morphology was induced by P. aeruginosa’s LPS for 72 h in BEAS-2B cells (c). The data represent the mean ± SEM, n = 3. *p < 0.05 versus the control