Fig. 3.

Blocking integrin αvβ6 could abrogate EMT in BEAS-2B cells induced by P. aeruginosa’s LPS. BEAS-2B cells were incubated with integrin αvβ6–blocking antibody 10D5 (30 μg/mL) or specific inhibitor of TGF-β1-Smad2/3 signaling, SB431542 (10 μM), for 2 h prior to incubation with 2 μg/mL P. aeruginosa’s LPS for 72 h. Western blotting showed that the decrease of the epithelial marker, E-Cad expression, and the increase of mesenchymal markers, Vi and α-SMA, induced by P. aeruginosa’s LPS were reversed by integrin αvβ6–blocking antibody 10D5 as well as SB431542 (a). Immunofluorescence showed similar results of E-Cad and Vi expressions (b). The data represent the mean ± SEM, n = 3. *p < 0.05 versus the control (Con), #p < 0.05 versus LPS