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. 2020 Feb 28;10:3767. doi: 10.1038/s41598-020-60775-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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quantitative PCR of neuronal and non-neuronal ChIP samples. (a) To validate neuronal and non-neuronal specificity of ChIP samples, samples were subjected to qPCR for GAPDH, GRIN2B and HBB which were also used in the optimization step (Fig. 1). (b) qPCR was also performed for SYN3 and BDNF, neuronal regions, and ERMN and OLIG2, non-neuronal regions. Fold enrichment was calculated using qPCR of enriched DNA fragment as ChIP/Input (%). The black bar represents mean ± SD. Statistical significance was determined by one-way ANOVA with post-hoc Turkey (H3K4me3 ChIP using neurons) and Brown-Forsythe ANOVA with post-hoc Dunnett’s correction (the others). The number of samples is shown in each panel. **P < 0.01, ***P < 0.001, ***P < 0.0001.