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. 2020 Feb 28;10:3715. doi: 10.1038/s41598-020-60431-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Intraperitoneal treatment of tgSOD1^G93A-ALS mice with 10 μg recombinant huANG post disease onset did not prolong survival. (a) Kaplan-Meier curves illustrated no significant differences in survival between tgSOD1^G93A-ALS mice treated with vehicle and tgSOD1G93A-ALS mice treated with 10 μg recombinant hANG (i.p., 3 times/week). Kaplan-Meier survival analysis was performed and the statistical significance of differences in survival between the categories was evaluated using Log-rank (p value 0.426), Breslow (p value 0.702), and Tarone-ware (p value 0.55) tests. A significance level of 0.05 was used.