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. 2020 Feb 28;10:3738. doi: 10.1038/s41598-020-60623-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Inhibitory zones of nisin A and nisin P against indicator strain L. bulgaricus LMG 6901. (B) Purification of nisin P from S. agalactiae DPC7040 and nisin H from S. hyointestinalis DPC6484 cultures. For nisin P: (B) RP-HPLC chromatogram and (C) MALDI-TOF MS of active fraction number 24. Expected mass for nisin P is 2991 Da. For nisin H: (D) RP-HPLC chromatogram and (E) MALDI-TOF MS of active fraction 30 showing mass corresponding to the expected nisin H mass of 3453 Da.