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. 2020 Feb 28;10:3738. doi: 10.1038/s41598-020-60623-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Chromatograms from LC-MS analysis of purified nisin P. (A) Elution gradient of 1–50% acetonitrile in 0.1% formic acid in 9 min and to 100% acetonitrile in 1 min. (B) Extracted-ion chromatogram (XIC) of nisin P. (C) Base peak intensity chromatogram. (D) Raw spectrum of series of charge states from 5+ to 2+. (E) Spectrum detail of charge state 4+, confirming expected mass of purified nisin P. The mass calculation is performed by multiplying by 4 the 747.8533 ion mass and removing four charges of protons (747.8533*4) − (4*1.007276) = 2987.3841.