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. 2020 Feb 28;11(2):155. doi: 10.1038/s41419-020-2338-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a lncRNA LDLRAD4-AS1 overlapped with the second intron of LDLRAD4. b lncRNA LDLRAD4-AS1 expression was distinctly negatively correlated with LDLRAD4 based on expression data from GSE39582 (Y = −0.0667*X + 3.947, p = 0.0026). c lncRNA LDLRAD4-AS1 expression was distinctly negatively correlated with LDLRAD4 based on PCR data in the 62 CRC tissues from FUSCC data set (Y = −0.3496*X + 1.250, p = 0.0052). d, e Expression levels of LDLRAD4 in CRC and colon mucosa epithelial (NCM460) cell lines using qRT-PCR analysis (d) and western blotting (e). f Expression levels of LDLRAD4 in 62 paired CRC and adjacent noncancerous tissues. g Kaplan–Meier analysis of cause-specific survival (CSS) in all patients with CRC, according to LDLRAD4 expression using qRT-PCR analysis (log-rank test). h Kaplan–Meier analysis of disease-free survival (DFS) in all patients with CRC, according to LDLRAD4 expression using qRT-PCR analysis (log-rank test). i, j Representative images show high LDLRAD4 expression in CRC tissues (i) compared with low LDLRAD4 expression in CRC tissues (j). k Kaplan–Meier analysis of cause-specific survival (CSS) in all patients with CRC, according to LDLRAD4 expression using IHC analysis (log-rank test). l Kaplan–Meier analysis of disease-free survival (DFS) in all patients with CRC according to LDLRAD4 expression (log-rank test) using IHC analysis. *p < 0.05, **p < 0.01, ***p < 0.001.