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. 2020 Feb 28;11(2):155. doi: 10.1038/s41419-020-2338-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a, b LDLRAD4-overexpressing RKO and LoVo cell lines were established by the transfection of pCDH-LDLRAD4. LDLRAD4 levels in cells were detected by qRT-PCR (a) and western blotting (b). c, d Migration assays were used to determine the effects of LDLRAD4 overexpression on the migration ability of CRC cells. e, f Invasion assays were used to determine the effects of LDLRAD4 overexpression on the invasion ability of CRC cells. g–j The migration potencies of CRC cells with the indicated treatments were detected by using wound-healing assay. k–l infecting DLD1 and HCT116 cells with a lentivirus vector harboring shRNA-LDLRAD4 was to knock down the endogenous expression of LDLRAD4 in cells. LDLRAD4 levels in cells were detected by qRT-PCR (k) and western blotting (l). m, n Migration assays were used to determine the effects of LDLRAD4-depleted on the migration ability of CRC cells. o, p Invasion assays were used to determine the effects of LDLRAD4-depleted on the invasion ability of CRC cells. q–t The migration potencies of CRC cells with the indicated treatments were detected by using wound-healing assay. For a–t, data were expressed as means ± SD in three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.