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. 2020 Feb 28;11(2):155. doi: 10.1038/s41419-020-2338-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Expression levels of LDLRAD4 in LDLRAD4-AS1-overexpression cells using qRT-PCR analysis. b Expression levels of lncRNA LDLRAD4-AS1 in LDLRAD4-overexpression cells using qRT-PCR analysis. c Expression levels of LDLRAD4 in LDLRAD4-AS1-overexpression cells using western blotting. d Expression levels of LDLRAD4 in LDLRAD4-AS1-depleted cells using qRT-PCR analysis. e Expression levels of lncRNA LDLRAD4-AS1 in LDLRAD4-depleted cells using qRT-PCR analysis. f Expression levels of LDLRAD4 in LDLRAD4-AS1-depleted cells using western blotting. g RNA fluorescence in situ hybridization (FISH) showed that lncRNA LDLRAD4-AS1 and LDLRAD4 were both located in the nucleus in close proximity to each other. h–k LncRNA LDLRAD4-AS1 overexpression disrupted stability of LDLRAD4 mRNA compared with the control group (h–i), whereas the stability of LDLRAD4 mRNA was obviously increased after lncRNA LDLRAD4-AS1 knockdown compared with the control group (j, k). l All five truncated mutants and lncRNA LDLRAD4-AS1 full length are illustrated. m Forced expression of three truncated mutants (TM-549-5′, TM-1098-5′, and TM-1098-M) significantly downregulated the levels of LDLRAD4 mRNA as lncRNA LDLRAD4-AS1 full length did. For a–m, data were expressed as means ± SD in three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.