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. 2020 Feb 28;5:17. doi: 10.1038/s41541-020-0166-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a In vitro exposure of PBMCs from most vaccine recipients to the down-selected panel of peptides (Table 2) for each structural protein resulted in upregulation of the T cell activation marker CD69 on both CD4 and CD8 T cells. The dotted line shows the background frequency based upon the highest frequency of expression observed in the negative control. Representative raw flow plots depicting CD69 expression are shown for the donor with the highest RVFV-specific T cell activity (RHI5). b PBMCs from RHI5 also expressed IFN-γ and TNF-α following peptide stimulation.