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. 2020 Feb 28;5:17. doi: 10.1038/s41541-020-0166-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Pentamers against 2 RVFV HLA-A2-directed epitopes (ILD and VLS) were labeled on both APC and PE and were incubated with PBMCs from various donors. A sample from a control donor who is HLA-A2-positive but unvaccinated is also included; this donor has influenza-specific CD8 T cells but no RVFV-specific CD8 T cells. The pentamer frequencies for each donor are shown in b. Dotted lines show the background value for each pentamer based upon the background signal observed in the HLA-A2-negative donor, RHI-7.