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. 2020 Feb 28;11:1128. doi: 10.1038/s41467-020-14811-1

Fig. 4. SPRING is essential for mouse embryogenesis and for hepatic SREBP signaling.

Fig. 4

a Illustration of the genomic organization of murine SPRING (2410131K14Rik-201) and the allele obtained after CRISPR/Cas9 editing. The 5’ and 3’ gRNAs are indicated in red and green, respectively. Sanger sequencing of amplified genomic DNA was performed to confirm the deletion of exons 2–5. b Table showing an overview of the obtained genotypes from various crosses of heterozygous mice. *p < 0,005, lower than expected by Pearson’s Chi-square test with 2 df; **equal to expected when assuming embryonic lethality of homozygous null mice, Chi-square = 2.37, 0.9 > p > 0.1 with 1 df. c WT C57BL/6J mice were administered Ad-shCtrl or Ad-shSpring (N = 8/5 animals per group, respectively) via tail-vein injection. After 7 days, mice were fasted overnight and subsequently refed for 6 h. Total liver RNA was isolated and expression of the indicated genes was determined by qPCR. Each individual mouse is plotted within the box and whiskers plot that depicts the median line, the 25th and 75th percentile, and the min-max values. *p < 0.05, ***p < 0.001.