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. 2020 Feb 28;10:3764. doi: 10.1038/s41598-020-60396-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Endoplasmic reticulum stress (ERS)-related protein expression in CT26 cells infected with KST0652. The CT26 cell monolayer was infected with KST0652 (MOI = 100) and irradiated with 2 Gy of γ-radiation at 2 h post-infection. (a) The expression levels of SspH1:sATF6_FLAG, p90ATF6, p50ATF6, GRP78, and CHOP proteins were detected using western blot analysis. β-tubulin was used as the loading control. Full-length blots are presented in Supplementary information. (b) SspH1:sATF6_FLAG was detected in KST0652-infected CT26 cells after 18 h post-irradiation. Immunofluorescence assay, followed by confocal microscopy, was used to visualize the location and expression of SspH1:sATF6_FLAG in CT26 cells. The nuclei of CT26 cells were stained with DAPI (blue), and SspH1:sATF6_FLAG were stained with FITC-conjugated anti-FLAG mouse IgG (green). White arrows indicate SspH1:sATF6_FLAG protein. Representative images are shown; original magnification, 100×. Changes in fluorescence signals of SspH1:sATF6_FLAG in CT26 cells. Data are mean fluorescence intensities (a.u.) ± SEM from three independent photos and asterisks (*) indicate significant difference between each group (** P < 0.01).