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. 2020 Feb 28;10:3760. doi: 10.1038/s41598-020-60638-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a–c) Hlf overexpression decreases the amplitude but does not affect the frequency of spontaneous currents under basal conditions. (a) Representative traces (sPSCs). (b) (left) Cumulative amplitude distributions for sPSCs. 64.3% of simulated samples had a p-value < 5%. Inset: Mean spontaneous currents. (right) Cumulative interevent interval distributions for sPSCs. 20.7% of simulated samples had a p-value < 0.05. (c) Density plot of sPSCs (left) amplitudes and (right) interevent intervals. (d) Decay kinetics of sPSCs. (e,f) Hlf overexpression reduces the frequency of spontaneous excitatory currents in the presence of bicuculline and glutamate (BIC+GLU). (e) Representative sPSCs traces. (f) (left) Cumulative amplitude distributions for sPSCs. 67.6% of simulated samples had a p-value < 0.05. (right) Cumulative interevent interval distributions for sPSCs. 72.6% of the simulated samples had a p-value < 0.05. Inset: Mean spontaneous currents. (g) Density plot of sPSCs (left) amplitudes and (right) interevent intervals. (h) No significant differences were observed in decay kinetics between groups (i,j) There are no differences in action potential number between neurons transduced with AAV-EGFP or a AAV-HLF in basal conditions or in the presence of bicuculline and glutamate. (i) representative action potential (AP) traces fired by a current step of 250 nA in (left) basal conditions and (right) in the presence of BIC+GLU. (j) Left panel: AP number. Right panel: AP amplitude. AAV-EGFP: black traces and dots. AAV-HLF: magenta traces and dots. Figures were created using Adobe Illustrator version 23.1.1., www.adobe.com/Illustrator; R version 3.4.3., https://www.R-project.org; Mini Analysis version 6.0.7., http://www.synaptosoft.com/MiniAnalysis/; Pclamp suite version 10, https://www.moleculardevices.com/products/axon-patch-clamp-system/acquisition-and-analysis-software/pclamp-software-suite#gref and GraphPad Prism version 8.3.1 for macOS, www.graphpad.com.