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. 2020 Feb 28;10:3665. doi: 10.1038/s41598-020-60291-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Representative histomicrographs of a buccolingual section at the experimental site: overview (A), gingival sulcus region (B), attached gingiva region (C), and mucosa region (D). The gingival sulcus and attached gingiva regions appear as keratinized, stratified squamous epithelial cell layers with numerous rete pegs and underlying dense connective tissue layer. In contrast, the mucosa region shows a nonkeratinized, thin epithelial layer and underlying loose connective tissue. Based on these specific histologic features, the mucogingival junction can be clearly distinguished and the keratinized tissue width (KW) can be measured.