Fig. 1. VLRB binding profiles of immune stimulated plasma screened on the CFG glycan microarray reveal that lampreys mount a targeted and diverse anti-glycan response against the given antigen.
Plasma was assayed at a 1:2 dilution for all samples except for the Tn4 B cells (f), which was screened at 1:5. Detection was achieved with an anti-VLRB mouse antibody, and goat anti-mouse Fc-488. The immunogens injected into the lamprey were a CHO—Pro.5, b CHO—Lec8, c CHO—Lec8GT, d CHO—Lec8GTFT, e Pig lung, f Tn4 B cells, and g human milk.