Fig. 3. Pairing yeast surface display and glycan microarray technology has led to the rapid identification of VLRB binding specificity and development of a novel enrichment strategy of yeast surface display libraries.

a Monoclonal VLRB RBC36 was expressed as a mouse Fc chimeric protein and screened on the CFG array. b Monoclonal VLRB RBC36 expressed on the surface of yeast retains the same binding specificity as the soluble protein. c Macroscopic images of yeast bound to the glycan microarray during the wash steps (top panel, left to right) and stained with an anti-Myc-488 antibody (bottom panel). d Three rounds of enrichment using the CFG array identified VLRBs which bound to distinct glycan epitopes.