Fig. 5. We have identified and characterized 12 monoclonal VLRBs from four YSD libraries which bind to glycans carrying the type 2 H-trisaccharide motif, and compared their specificity with previously reported type 2 H VLRBs and the plant lectin UEA-I.

The RFU values were normalized to a scale of 0–100 and are shown in the form of a heat map (a). The RFUs are plotted for two VLRBs, Tn4-27 that preferentially binds to the type H antigen on N-glycan presentations, and Tn4-21 that binds to O-glycan presentations of the antigen (b).