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. 2020 Feb 28;11(2):154. doi: 10.1038/s41419-020-2348-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Relative Nr5a2 mRNA expression in the liver, spleen, and bone marrow-derived macrophages (BMDM) from wild-type C57BL/6 mice, as well as in the macrophage cell line RAW 264.7 (RAW). Results are shown as relative to murine Gapdh mRNA expression. Mean values (bars) and individual data points from three mice, resp. cell samples, per group are shown. b Endogenous LRH-1-dependent transcriptional activity in RAW 264.7 cells. Cells were transfected with the LRH-1 luciferase reporter construct (5 × RE LRH-1) or an empty luciferase reporter (pGL3) and treated with vehicle (PBS) or 3d2 (40 μM). Mean values of triplicates ± SD of two independent experiments are shown. Luciferase activity was normalized to untreated cells (t test; **p < 0.01). c TNF protein levels measured in the supernatant of splenocytes from wild-type C57BL/6 mice and pre-treated for 2 h with indicated concentrations of 3d2, prior to stimulation with control buffer (CTL) or LPS (30 ng/ml). Mean values of triplicates ± SD of a representative experiment (n = 3) are shown (one-way ANOVA, ***p < 0.001).