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. 2020 Feb 28;11:1112. doi: 10.1038/s41467-020-14916-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a CpG methylation along three representative SEs in embryonic stem cells (ESCs; serum/LIF)¹¹ and epiblast stem cells (EpiSCs; this study) in CDM supplemented with activin A and fibroblast growth factor 2 as represented in Fig. 1a. Unmethylated SE subregions that gain methylation in EpiSCs are represented as magenta bars (DM subregions), and those that remain unmethylated as green bars (PU subregions). Grey bars represent interstitial (INT) regions in ESCS that remain methylated in EpiSCs. b Proportion (%) of SE subregions in ESCs and EpiSCs. Unmethylated regions in ESCs (blue bar) either remain unmethylated (PU, green, 184 subregions) or become methylated (DM, magenta, 492 subregions) in EpiSCs; INT (547 subregions) methylated regions are shown in grey. A total of 231 SEs were analysed. c CpG methylation levels (mCpG/CpG) at PU, DM and INT subregions in an independent ESC line grown in serum/LIF (ser)¹⁴, in EpiLCs and an independent EpiSC line²³. ESCs grown in 2i/LIF (2i)¹¹ are also shown; 2i/LIF conditions enforce a globally hypomethylated state of genome. d CpG methylation levels (mCpG/CpG) at PU, DM and INT subregions in vivo in pre-implantation (inner cell mass (ICM); E3.5 and E4.0) and post-implantation epiblast (Epi; E5.5 and E6.5) tissues²².