Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 28;10:3684. doi: 10.1038/s41598-020-60680-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

P334 mediates efficient generation of iPSCs from mouse somatic cells. (A) Schematic diagram describing the main theme and experimental process. (B) Representative AP (alkaline phosphatase) staining images of the generated iPSCs with and without P334 treated at 0 to 15 days after dox induction. Three independent experiments of three sets each were performed with 10 visual fields per set. (Scale bar: 200 um) (C) Bar graph showing the number of AP positive colonies with and without P334 treated at 0 to 15 days after dox induction (data represent mean ± SEM. *p < 0.05, **p < 0.01). (D) Immunostaining showing the expression of pluripotency markers, OCT3/4 and NANOG in each condition (control and P334 treated). (Scale bar: 100 um) (E) Bar graph representing number of OCT3/4 and NANOG positive cells per field (data represent mean ± SEM. **p < 0.01). (F) Bar graph demonstrating the size of iPSCs in each condition (control and P334 treated) (data represent mean ± SEM. **p < 0.01).