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. 2020 Feb 28;10:3684. doi: 10.1038/s41598-020-60680-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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P334 mediated mouse iPSCs can be differentiated to three germ layers. (A) Mouse embryonic body (EB) based differentiation from P334 mediated iPS cells. Left panel showing the brightfield image of differentiated iPS cells. Right panel showing the immunostaining analysis of three germ makers in the differentiated P334 mediated iPS cells. Ectoderm: TUJ and NESTIN, Endoderm: SOX17 and Mesoderm: BRACHYURY. 15dpi iPSCs were used for EB generation and cultured extra 10 days after EB attachment process. (Scale bar: 100 um) (B) EB based differentiation from P334 mediated iPS cells to osteoblasts (marker: OSTEOCALCIN) and dopaminergic neuron (marker: TH). 15dpi iPSCs were used for differentiation and cultured extra 20 days after starting differentiation process. (Scale bar: 30 um).