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. 2020 Feb 28;10:3701. doi: 10.1038/s41598-020-60619-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gallery of Cryo-TEM images of urinary EVs recovered in the relative low centrifugation pellet P21 before and after TCEP reduction (P21^TCEP). A heterogeneous population of uEVs was observed including single layered vesicles and multiyered structure with two or more inner small vesicles encapsulated inside bigger vesicles before (A–F) and after TCEP treatment (G1–G6). Tamm-Horsfall protein (THP) long polymeric filaments (indicated by arrows) (A) either engulfing (*) (C) or adsorbing (#) vesicles (B and b1) were also visible. No filaments of THP were visible (G1–G6) after TCEP reduction.