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. 2020 Feb 28;10:3701. doi: 10.1038/s41598-020-60619-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Particle size distribution and concentration. (A) Tunable resistive pulse sensing of P21 pellet; (B) Nanoparticle tracking analysis (NTA) of P21 pellet; C NTA of P21^TCEP pellet and (D) P21^TCEP supernatant. Black marks represent the particle concentration of re-solubilized pellet, red marks refer to the particles concentration per mL of urine. X- axis represent volume of urine (mL) processed to obtain the uEV pellet. Pellets for TRPS were re-solubilized in 100 μL of PBS-0.1μm (A). Pellets for NTA were resolubilized in 200 μL of 10 mM Tris-HCl pH8.8 + 4 mM TCEP- for P21 (B) and P21^TCEP (C) pellets and 1.2 mL 110 mM Tris-HCl pH8.8 + 4 mM TCEP for SN21^TCEP (D).