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. 2020 Feb 28;10:3701. doi: 10.1038/s41598-020-60619-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and western blot (WB) analysis of P21 pellet. The whole pellet - originated from different volumes - was loaded in each lane and stained with either silver nitrate (A) or colloidal Coomassie (B,C). Nitrocellulose membranes were hybridized respectively with: collectrin (TMEM27) (D) and tissue inhibitor of metalloproteinases 2 (TIMP-2) and CD9. After the first image acquisition the same membranes were incubated again with insulin-like growth factor binding protein 7 (IGFBP-7) (G), podocin (NHPS2) (H) and calreticulin (CALR) (I) respectively. Finally membranes were incubated a 3^rd time with tumor susceptibility gene 101 (TSG101) (J), podocalyxin (PODXL) (K) and calnexin (L) respectively. Samples for gel in C and western blots in (F,I,L) were run without DTT. K rat kidney, S Saliva pellet 4.600 g.