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. 2020 Feb 28;10:3701. doi: 10.1038/s41598-020-60619-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Imaging flow cytometry quantification. Concentration obtained by gating positive events (Supplementary Fig. S13) of: (A) IGFBP7; (B) TIMP2; (C) TMEM27; (D) AnnexinV; (E) Autofluorescence emission in channel 5; (F) Auto fluorescence emission in channe 11; (G) PODXL (F) PODXL without autofluorescence excluded applying the Boolean or logic function: “Not AF gate Ch5 And Not AF gate Ch11”. Black marks particles concentration of re-solubilized pellets, red marks particles concentration per mL of urine. X-axis shows the volume of urine (mL) processed to obtain the uEVs pellet.