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. 2020 Feb 28;10:3701. doi: 10.1038/s41598-020-60619-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Imaging flow cytometry quantification single staining for PODXL. Co-detection of PODXL and AF emission in channel 11 at low (A), medium (B) and high (C) scatter based on the gate strategy in Supplementary Fig. S19. Application of morphology (D) and intensity (E) masks combined with the spot count feature show the number of coincidence event. Yellow digits in the image gallery indicate the number of events calculated by the spot feature.