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. 2020 Feb 28;10:3701. doi: 10.1038/s41598-020-60619-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Real time quantitative PCR (qPCR) analysis of P21 pellet. RNA from the whole P21 pellet - originated from different volumes - was isolated and reverse-transcribed. qPCR analysis for miR-16 (blue), miR-155 (red), miR-200b (gray), and miR-203 (yellow) was performed and the relative cycle threshold values (dCt) were plotted for each of the volumes. The trend line with the R² value indicates that the amount of miRNA increased with the increased volume of urine used for processing the pellet.