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. 2020 Feb 28;10:3701. doi: 10.1038/s41598-020-60619-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SDS-polyacrylamide gel electrophoresis (SDS-PAGE), western blot (WB) analysis and particles concentration of P21, P21^TCEP pellets and SN21^TCEP supernatants after TCEP reduction in healthy donors. Pellets and TCEP supernatant originated from 9 mL of urine from 4 female (lane1-4) and 4 male (lane 5–8) were loaded in each lane and stained with colloidal Coomassie or hybridized with anti tumor susceptibility gene 101 (TSG101) for P21 pellets (A,B), P21^TCEP pellets (C,D) and SN21^TCEP supernatants (D,F). Particle concentration measured with NTA (G) for P21 pellets (light gray), P21^TCEP pellets (grey) and SN21^TCEP supernatants (black).