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. 2020 Feb 28;3:89. doi: 10.1038/s42003-020-0821-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Eleven carotenoids and four cyanins were identified and quantified from the sepals, petals, and lip segments at D7 and D8 by using an LC-ESI-MS/MS system. The highest carotenoid and cyanin contents were detected in the hypochile and epichile, respectively. From D7 to D8, the total chlorophyll content was reduced in all segments. Sepal, petal, epichile, and hypochile are abbreviated as Sep, Pet, Epi, and Hyp, respectively. The data are the mean ± SD from three biological replicates. Asterisk and double asterisk indicate values that differ significantly from those in the segment with the highest pigment content in every stage at P < 0.05 and P < 0.01, respectively, according to Student’s paired t-test. b The expression profile of ABP structural genes in the sepals, petals, and lip segments from D1 to D8. c The expression profile of CBP structural genes in the sepals, petals, and lip segments from D1 to D8. The data are the mean of three biological replicates. Isoform and unigene are abbreviated as Is and Ug, respectively. CHS Chalcone synthase; CHI chalcone isomerase; F3H flavanone 3-hydroxylase; F3′H flavonoid 3′-hydroxylase; DFR dihydroflavonol 4-reductase; ANS anthocyanidin synthase; BZ1 flavonoid glucosyltransferase; PSY phytoene synthase; PDS phytoene desaturase; Z-ISO ζ-carotene isomerase, ZDS ζ-carotene desaturase; CRTISO carotenoid isomerase; LCYE lycopene ε-cyclase; LCYB lycopene β-cyclase; BCH β-carotene hydroxylase; CYP97A3 P450-type β-ring hydroxylase; CCD carotenoid cleavage dioxygenase.