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. 2020 Feb 28;3:89. doi: 10.1038/s42003-020-0821-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Phylogenetic trees based on the neighbour-joining method were inferred from the amino acid sequences of KOVA AP3- and AGL6-like MADS-box proteins and those of other plants (left). According to the FPKM values of these KOVA MADS-box genes, their expression profiles in flower segments during floral development were described via heatmap analysis (right). The FPKM value of each isoform/unigene is the mean of three biological replicates. b The brown module shows that AP3-1s and AP3-4s presented a co-expression relationship with some CBP structural genes during floral development according to WGCNA. c Sampling strategy for the sepals and petals at D4 (left) and D7 (right), which was similar to the sampling strategy for the lip. These samples were used for qRT-PCR analysis to explore the AP3- and AGL6-like MADS-box gene expression profiles. d The qRT-PCR results showed that the differences in the expression levels of the AP3- and AGL6-like genes between the perianth segments were smaller than those in the lip segments. The data are the mean ± SD from three biological replicates.