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. 2020 Feb 28;11:1114. doi: 10.1038/s41467-020-14921-w

Fig. 6. Homing of lymph-derived activated CD4 T cells into LN parenchyma depends on CCR7.

Fig. 6

Representative images (left) and quantitative analysis (right) of adoptively transferred cells in popliteal LNs 90 min after i.l. injection of 1:1 mixtures of activated CD4+ T cell combinations as indicated; a in vitro-activated wild-type (wt) and Ccr7−/−; b in vitro-activated PTX-treated Ccr7−/− and PBS-treated Ccr7−/−; c in vivo-activated DO11.10 and DO11.10Ccr7−/−; d in vivo-activated PTX-treated OTII and PBS-treated OTII cells (left; green and red, cells; blue, counterstaining with anti-Lyve-1, scale bars 100 µm). Middle panels, total cell counts in the indicated compartment; dots represent cell counts per LN section; numbers above indicate the percentage of change compared to control cell population; (parench parenchyma; sin. sys sinus system). Right panels, migration distance from the SCS; dots represent cells; red bars, median; ns, not significant; Wilcoxon signed rank test **p < 0.01; ***p < 0.001. Data are derived from four experiments with a total of 12 (a) or 7 (c) mice, or from three experiments with a total of eight (b) or five (d) mice.