Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 28;11:1114. doi: 10.1038/s41467-020-14921-w

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — Quantitative analysis of adoptively transferred cells in popliteal LNs 90 min after i.l. injection of combinations of activated CD4⁺ T as indicated: a wild type and 4Itg^−/−; b wild type and Talin-1^−/−. a, b Left, total cell counts; dots represent cell counts per LN section; numbers above indicate the percentage of change compared to wild type (parench parenchyma, sin. sys. sinus system). c Migration distance of adoptively transferred cells from the SCS as indicated; dots represent cells; red bars, median; ns, not significant; *p < 0.05; **p < 0.01; ***p < 0.001 (paired t-test). Analysis of intranodal CD4⁺ T cell migration (left) and representative movies (right) (d–f) based on ex vivo time-lapse imaging of popliteal LNs after i.l. injection of activated wild type and Talin1^−/− (d); wild type and 4Itg^−/− (e), or PTX-treated wild type and 4Itg^−/− (f) CD4⁺ T cells. Data calculated based on all cell tracks present within selected regions using automated tracking with manual correction. Dots represent the mean of one cell population per movie. ns not significant; a, b Wilcoxon signed rank test; c Mann Whitney test *p < 0.05; **p < 0.01, and ***p < 0.001 (paired t-test). Data are derived from three experiments with a total of eight (a, c) or four (d–f) mice, or from two experiments with a total of six mice (b, c). Scale bars 50 µm.